Genekam adipogenic differentition media (Sterile Product)*
Prepare the media while adding 10 ml of Genekam adipogenic differentiation media into 90ml of DMEM containing 10 % FCS + 1% SP + 1% Glutamine or in your culturing media. Store it at 4°C. You can prepare less media, but the ratio should be 1:10.
- After you have stem cells e.g. MSC. To isolate MSC cells, one can use Genekam Stem cell isolator kit. After you get 60-85% confluence during culturing of stem cells, please remove the cell using trypsin or Genekam cell remover and wash the cells to make free from trypsin while Centifuging them at 1400 rpm for 5 minutes and suspend the pellet now to make the cell count using trypan blue.
- Now add the cells 1 x 104 / cm2 on a plate e.g. 9 or 12 wells in adipogenic differentiation media (containing DMEM + 10 % FCS or other serum source + Genekam adipogenic differentiation media) for two weeks while feeding the cells 3-5 days with this media.
- After two weeks, you can stain with cells with red O stain to see the intracellular lipid accumulation.