from German Federal Ministry of Economics and Technology.
Neural cell differentition of MSC (Steril Product)*
Kit contents: STEM07 Neural cell differentition of MSC: 10X = 10 ml (store at -20°C)
Final Neural cell differention media: Add 10 ml of Genekam neural cell differentiation media into 90 ml of DMEM + 15% FCS + 1% SP + 1% Glutamine or in your culturing media. Store it at 4°C. You can prepare less media, but the ratio should be 1:10.
- After you have stem cells e.g. MSC. To isolate MSC cells, one can use Genekam Stem cell isolator kit. After you get 60-85% confluence during culturing of stem cells, please remove the cell using trypsin or Genekam cell remover and wash the cells to make free from trypsin while Centifuge at 1400 rpm for 5 minutes and suspend the pellet now to make the cell count using trypan blue.
- MSC were seeded on glass coverslips coated with 1 mg/ml p-lysine and 13 ug/ml laminin in final neural cell differentiationmedia for 4 weeks with feeding interval of 3-5 days with final media. One can do immunostaining against gamma amino butyric acid (GABA) and tyrosine-hydroxylase (TB).