Technical details for realtime PCR kit Chlamydia pneumoniae

FR003 Chlamydia pneumoniae, 100 reactions

Chlaymdia pneumoniae is known to cause the respiratory as well as reproductive diseases in human beings and its role is cardiovascular diseases like atherosclerosis, myocarditits is being discussed in many publications in the near past. Chlamydiae can be detected through staining methods, cell culture methods as well as serological methods like FAT, CFT etc, but these all methods have one or other following drawbacks: low sensitivity, cross reactions or need a long time for detection, therefore PCR is merging as godstandard as this is highly sensitive and finished within a few hours. With real time PCR, one can get the results within 3-4 hours as there is no post PCR stage. In case one can run the known standards with PCR, it can give you the exact number of copies present in the sample. In case the quantititive standard is not available, one can compare 2 samples as one see the chlamydia load as curve during real time assay. This micro organism has been detected in animals and there is a need of more research for the role of this bacteria in animals. Real time PCR can be used during the course of treatment as well as to see the pharmokinetics of the drug in vitro against the Chlamydia pneumoniae. During the treatment, if the bacteria load is not going down, it is the indication of the change of line of treatment.

target gene: highly conserved gene of Chlaymdia pneumoniae

Samples: blood, respiratory swabs, serum, genital swabs, heart and related blood vessel samples, tissue and other body fluids.It needs the isolated DNA, which can be isolated through commercial kits or home made methods.

Validation: It has been checked for cross reactions for related and non related micro organisms like Chlaymdia trachomatis, Chlaymdia psittaci, Epstein Barr virus, Staphylococcus aerus, Mycoplasma pneumoniae, uninfected human DNA, Mycoplasma hominis, Legionella and many more. It has been checked for serial dilutions of Chlamydia pneumoniae.

Detection limit: minimum 50 copies per assay (detectio Ct is lower 36 (this should be taken as positive); 36-40 Ct should be taken as weak positive.)

Contents of the kit: Polymerase, buffer, PCR master mix, nucleotides, primers, probes, positive control, negative control, molecular water and manual.

Storage: -20 degree

- only for in vitro use
- only for research use
- to be used through technical person

manual

Last modified:
2012-04-05 12:46:27