from German Federal Ministry of Economics and Technology.
Technical details for realtime PCR kit Human hepatitis B virus
FR007 Human hepatitis B virus, 100 reactions
Human Hepatitis B Virus (HBV) is one of the most frequently parenterally transmitted viruses and needs special diagnostic assays as well as safety steps for blood and blood products. Quantitive assays should cover wide range of concentrations since the absolute viral load as well as its time course are important diagnostic parameters for indicating the prognosis as well as therapy. HBV can be detected through cell culture methods as well as serological methods like ELISA etc, but these all methods have one or other following drawbacks: low sensitivity, cross reactions or need a long time for detection, therefore PCR is merging as godstandard as this is highly sensitive and finished within a few hours. With real time PCR, one can get the results within 3-4 hours as there is no post PCR stage. In case one can run the known standards with PCR, it can give you the exact number of copies present in the sample. In case the quantititive standard is not available, one can compare 2 samples as one see the HBV load as curve during real time assay. Real time curves can be helpful during the antiviral therapy and predict the success of the treatment.
In case the viral load is going down, it means that the therapy is going to be successful, otherwise one has to think about other alternatives.
target gene: highly conserved region of HBV gene
Samples: blood, serum, plasma, cell culture, tissue and other body fluids. This needs isolated DNA, which can be isolated through commercial DNA isolation kit.
Keypoints : It has been checked for cross reactions for related and non related micro organisms.
Detection limit: minimum : 100 genome equivalent per assay (detection Ct is lower 36 (this should be taken as positive); 36-40 Ct should be taken as weak positive.)
Contents of the kit: Polymerase, buffer, PCR master mix, nucleotides, primers, probes (with 6-carboxyfluoresceine and 6-carboxytetramethylrhodamine), positive control, negative control, molecular water and manual.
Storage: -20 degree
Recommendations: always run the two samples per probe.
- only for in vitro use
- only for research use
- to be used through technical person.