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European community FP7

(24.01.2014)
Genekam Biotechnology AG has been selected for business mission trip to Tokyo, 26-30 May 2014 under EU GATEWAY TO JAPAN-Healthcare and Medical technologies. Genekam Biotechnology AG is one of 40 companies selected across the European Union.

(13.06.2012)
Genekam got one project for development of Nanoassays for nanomedicine under program ZIM
GENEKAM Biotechnology AG from German Federal Ministry of Economics and Technology.

(02.01.2012)
Genekam is to take part in European Community FP7 ... read more...

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ArabLab The Expo 2014

Visit us in Dubai, UAE from 17.03 to 20.03.2014
arablab.com

26. CHINA MED 2014

Visit us in Beijing / VR China 21.03. - 23.03.2014
chinamed.net.cn

Medica Düsseldorf 2014

Visit us on Medica 2014 in Düsseldorf from 12.11 to 15.11.2014
medica.de

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New Catalog 2014

Our new Catalog is now released, please download klick here

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Hard fact - is it a sign of quality and confidence in our product?

... in 2004 we had clients in 5 countries in whole year and in 2009 we served 60 countries of the world? There is a surge of medical publications where Genekam PCR Kits are being used. Is it not a sign of quality and trust ?
read more...

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Real time PCR Kits for Influenza A and swine flu (H1N1) (click here to see more information) read more...

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In vitro immunisation of human b-lymphocytes and human monoclonal antibodies read more...

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Genekam Solutions for combating SARS viruses read more...

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Genekam 3D-Stemcell-Solutions read more...

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Genekam 解决方案对抗SARS病毒 read more...

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Genekam-Barcode: Biotech for nature and endangered species
(A new DNA-Test to know the exact species of an animal) read more...

Technical details for realtime PCR kit Human herpes simplex virus



FR018 Human Herpes Simplex Virus 1, 100 reactions

Standard laboratory technique for herpes detection is cell culture as one recognise the virus through cytopathic affects as well as later on serological detection. HSV 1 and 2 infections overlap in many clinical samples and these viruses have been isolated from skin, mucosa and visceral organs. Many studies have shown that PCR is the reliable method to detect the herpes viruses as well as other viruses. Moreover PCR is the fastest method as the results with the real time PCR can be finished within 3-4 hours. Moreover one can see the load of the virus in real time PCR and this can be monitored during the preventative measurements.

Target gene: highly conserved region of g region of HSV 1 and 2

Kind of probes: body fluids like saliva, blood (serum), tissue biopsies, nerve tissues, genital biopsies. This kit needs DNA, hence it should be isolated with good quality commercial kit or home made method. Genekam Biotechnology AG can ship you the 3rd party DNA isolation kit.

Key points: The kit has been checked for cross reactions with different herpes viruses like EBV, CMV and of animal origin like Equine Herpes viruses, Koi´s Herpes virus etc. and with non related micro organisms. They have been used for the different dilutions and for the sensitivity.

Detection limit: 10-50 genome copies of HSV1 and HSV2

Components of the kit:

Contents of Kit: primern, fluorogenic primers (filter FAM to used in one step PCR and filters FAM and VIC are to be used in 2 step PCR), nucleotides, buffers, molecular water, negative control, positive control and manual. Dyes used are 6-carboxy-fluorscein, YYE and 6-carboxytetramethylrhodamine.

Storage: -20 degree

- only for in vitro use
- only for research use
- to be used through technical persons



 

Last modified:
2012-04-05 13:01:27