Technical details for realtime PCR kit Mycobacterium tuberculosis SINGLE CHECK

FR012A Mycobacterium tuberculosis SINGLE CHECK, 100 reactions

Tuberculosis (TB) is one of major public health problem throughout the world. In order to overcome this disease, one has to develop rapid, sensitive and specific identifcation methods. Traditional laboratory methods such direct microscopy and culture methods have drawbacks in view of identification of Mycobacterium tuberculosis as they are not so sensitive or they need skilled manpower (who give again different results of the same probe) or need a long time. The detection of Mycobacterium tuberculosis DNA sequence might be representing a more sensitive and fast daignostic target. One needs efficient method to detect the Mycobacterium tuberculosis in sputum and moreover to monitor the drug therapy, there is need of quantitative PCR assay. At present, the laboratories are using the PCR assay, which detects only one target gene and there are a certain strains of Mycobacterium tuberculosis, which does not contain this strain, therefore they can escape this test, therefore Genekam has brough a ready to use PCR kit, which can detect one most common gene target (which is widely accepted as standard throughout the world in all laboratories carrying the identification of Mycobacterium tuberculosis) and an extra gene, which is available in certain strains without the most common gene target.

However Genekam offers the assay in two forms:
1. ready to use kit for one target gene
2. ready to use kit for detectiong 2 different target genes

target gene: one IS gene (multicopy gene) in simplex version and two highly conversed gene targets (one is IS: it is multicopy gene and an other gene) in duplex version

Types of the samples: Sputum, strains from the culture, Respiratory fluid, lung, tissue, blood etc. DNA can be isolated through commecial available kits or home made good methods. This kit need good quality of DNA.

Contents of Kit: primern, fluorogenic primers (filter FAM to used in one step PCR and filters FAM and VIC are to be used in 2 step PCR), nucleotides, buffers, molecular water, negative control, positive control and manual. Dyes used are 6-carboxy-fluorscein, YYE and 6-carboxytetramethylrhodamine.

Validations: The assay has been validated for cross reactions with related like Mycobacterium avium sp paratuberculosis, Mycobacteriu avium sp avium etc and non related micro organisms like Salmonella, Legionella, Staphyloccus etc. along with the different viruses of human and animal origin. The assy is 98% sensitive in most common used IS gene against the microscopic method.

detectionlimit: 10-50 genome equivalent per reaction

Storage: -20 degree

- only for in vitro use
- only for research use
- to be used through technical person

Last modified:
2012-04-05 13:03:05